Analysis and mapping quantitative trait loci for histidine content in barley (Hordeum vulgare L.) using microsatellite markers

Abstract Mining the gene of histidine content in barley grain helps with breeding functional varieties. The study constructed a recombinant inbred lines (RILs) containing 193 families derived from cross between Ziguangmangluoerling (ZGMLEL) (?) and Schooner No.3 (?). (HIS) mapping population its parents were determined by an automatic amino acid analyzer. HIS ZGMLEL was 0.53 mg/g. No. 3 0.21 mg/g, ranged 0.23 to 0.54. Genetic linkage maps, including those seven chromosomes barley, using 180 pairs simple sequence repeat (SSR) markers, total genetic distance 2671.03 cM average marker spacing 14.84 cM. Quantitative trait locus (QTL) IciMappingV3.3 used analyze QTL grains, three QTLs detected. Mapping results showed that loci located on 2H, 4H, 7H, respectively. major contribution rate 10.11% chromosome 4H (HVBAMMGB84-BMAG0808). additive effect is positive (0.025). Thus, it comes high-value parent ZGMLEL. Another 13.75% 7H (GBM1303-GMS056). minor 6.01% 2H (Scssr03381-Scssr07759). effects negative (? 0.02 ? 0.033). So, they came male Schooner. provided reference for further fine mapping, cloning, transformation genes grains.